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mirna  (MedChemExpress)
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a) Volcano plot representation of <t>miRNA</t> expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis
Mirna, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a) Volcano plot representation of <t>miRNA</t> expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis
Igg Negative Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a) Volcano plot representation of <t>miRNA</t> expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis
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non targeting negative control sirna  (Sangon Biotech)
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SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
Non Targeting Negative Control Sirna, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nc mimic  (MedChemExpress)
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SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
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negative control nc sirna  (Sangon Biotech)
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SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
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negative control shrna sh nc  (Genechem)
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SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
Negative Control Shrna Sh Nc, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit igg  (EpiCypher)
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SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after <t>siRNA-mediated</t> knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.
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Image Search Results


a) Volcano plot representation of miRNA expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis

Journal: bioRxiv

Article Title: Microglia-derived extracellular vesicles attenuate acute a-synuclein induced astrocyte inflammation

doi: 10.64898/2026.05.11.724371

Figure Lengend Snippet: a) Volcano plot representation of miRNA expression differences between microglia-derived VEVs and REVs following miRNA sequencing analysis; Upregulated genes (red) and down regulated genes (blue) with Log 2 foldchange>4 and Log 2 p value>4 for significance b) KEGG pathway analysis of miR-5099 associated target proteins determined via miRNA target analysis

Article Snippet: Cells were transfected 24 hours prior to additional treatments. miRNA mimics for mmu-miR-5099 (MED Chem express, Catalog# HY-R03245), mmu-miR-720 (Thermofisher, Catalog # 4427975) which has the same mature sequence as target Novel-miRNA-115 identified though miRNA sequencing, and scrambled miRNA (MCE, Catalog # HY-R04602 Lot#825593) were utilized for the transfections.

Techniques: Expressing, Derivative Assay, Sequencing

SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.

Journal: Nucleic Acids Research

Article Title: In situ tracking of glycoRNAs on single-cell surface to reveal RNA heterogeneity and transport mechanism

doi: 10.1093/nar/gkag362

Figure Lengend Snippet: SNARE protein knockdown verification and colocalization analysis with glycoRNA. ( A ) Relative mRNA expression of v-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( B ) Protein expression levels of v-SNARE protein were detected by Western blot. ( C ) Quantitative analysis of Western blot result( B ). ( D ) Relative mRNA expression of t-SNARE protein after siRNA-mediated knockdown was determined by quantitative real-time PCR (qPCR). ( E ) Protein expression levels of t-SNARE protein were detected by Western blot. ( F ) Quantitative analysis of Western blot result ( E ). Data are presented as mean ± SD ( n = 3). ( G ) Co-localization of t-SNARE (TSNARE1 channel) and v-SNARE (VTI1B channel) with GLINT-labeled glycoRNAs in the images (FAM channel). ( H ) The plot profiles show co-localization of glycoRNAs with t-SNARE or v-SNARE.

Article Snippet: siRNAs targeting human VTI1B, TSNARE1, and GAPDH, as well as a non-targeting negative control siRNA, were synthesized by Sangon Biotech (Shanghai, China).

Techniques: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Labeling